DIF-3 is a bioactive anticancer compound that can downregulate cyclin D1 and c-Myc by facilitating their degradation via activation of GSK-3β. Also inhibiting the Wnt/β-catenin signaling pathway-related proteins in DLD-1 cells.

Physicochemical Properties

Molecular Formula	C13H17CLO4
Molecular Weight	272.72468
Exact Mass	272.082
CAS #	113411-17-9
PubChem CID	3081033
Appearance	White to off-white solid powder
Density	1.24g/cm3
Boiling Point	416.9ºC at 760mmHg
Flash Point	205.9ºC
Vapour Pressure	1.53E-07mmHg at 25°C
Index of Refraction	1.551
LogP	3.522
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	6
Heavy Atom Count	18
Complexity	272
Defined Atom Stereocenter Count	0
InChi Key	JWBMZIJMSBFBIY-UHFFFAOYSA-N
InChi Code	InChI=1S/C13H17ClO4/c1-3-4-5-6-8(15)11-9(16)7-10(18-2)12(14)13(11)17/h7,16-17H,3-6H2,1-2H3
Chemical Name	1-(3-chloro-2,6-dihydroxy-4-methoxyphenyl)hexan-1-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Differentiation-inducing factor-3 inhibits intestinal tumor growth in vitro and in vivo. J Pharmacol Sci. 2015 Apr;127(4):446-55.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~366.68 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (9.17 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.6668 mL	18.3338 mL	36.6676 mL
	5 mM	0.7334 mL	3.6668 mL	7.3335 mL
	10 mM	0.3667 mL	1.8334 mL	3.6668 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.