CM10 is a potent and specific aldehyde dehydrogenase 1A (ALDH1A) family inhibitor (antagonist) with IC50s of 1700, 740 and 640 nM for ALDH1A1, ALDH1A2 and ALDH1A3 respectively. CM10 has no inhibitory activity against other members of the ALDH family. CM10 regulates metabolism and has anti-cancer activity.

Physicochemical Properties

Molecular Formula	C20H23N3O
Molecular Weight	321.416124582291
Exact Mass	321.184
CAS #	692269-09-3
PubChem CID	889692
Appearance	Light green to green solid powder
Density	1.12±0.1 g/cm3(Predicted)
Boiling Point	512.4±52.0 °C(Predicted)
LogP	4.8
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	7
Heavy Atom Count	24
Complexity	402
Defined Atom Stereocenter Count	0
InChi Key	ZFHWEWJDCFGRBO-UHFFFAOYSA-N
InChi Code	InChI=1S/C20H23N3O/c1-3-8-15-9-7-10-16(19(15)24)14-21-20-22-17-11-5-6-12-18(17)23(20)13-4-2/h3,5-7,9-12,24H,1,4,8,13-14H2,2H3,(H,21,22)
Chemical Name	2-prop-2-enyl-6-[[(1-propylbenzimidazol-2-yl)amino]methyl]phenol
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	CM10 (12.5, 25, 50, and 100 μM; 1 hour) selectively kills CD133+ late-stage cancer stem-like cells (CSC) and suppresses ALDEFLUOR activity in living cells [1]. Tumor flow pattern in cells and nuclei is induced by CM10 [1].
References	[1]. A Pan-ALDH1A Inhibitor Induces Necroptosis in Ovarian Cancer Stem-like Cells. Cell Rep. 2019 Mar 12;26(11):3061-3075.e6.

Solubility Data

Solubility (In Vitro)

DMSO : ~125 mg/mL (~388.90 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (6.47 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.47 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.1112 mL	15.5560 mL	31.1119 mL
	5 mM	0.6222 mL	3.1112 mL	6.2224 mL
	10 mM	0.3111 mL	1.5556 mL	3.1112 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.