C3a (70-77) TFA (Complement 3a (70-77) TFA) is an octapeptide corresponding to the COOH terminus of C3a and displays C3a specificity and 1 to 2% bioactivity.

Physicochemical Properties

Molecular Formula	C37H62F3N13O12
Molecular Weight	937.96
Related CAS #	C3a (70-77);63555-63-5
Appearance	Solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

Human mononuclear leukocytes in culture that are directly interacting with C3a(70–77) exhibit concentration-dependent suppression of the synthesis of LIF elicited by mitogens and the antigen SK-SD[1]. C3a(70–77) increases vascular permeability in human skin, stimulates contraction of guinea pig ileal tissue, and causes histamine release and degranulation of rat mast cells. Ileal smooth muscle is desensitized to C3a(70–77) but not to human C5a, a similar anaphylatoxin. Cross-desensitization suggests that C3a(70–77) interacts with cellular C3a binding sites in a particular way[2].

References

[1]. Modulation of human lymphocyte function by C3a and C3a(70-77). J Exp Med. 1982 Sep 1;156(3):756-65. [2]. The active site of C3a anaphylatoxin. J Biol Chem. 1980 Nov 25;255(22):10758-63.

Solubility Data

Solubility (In Vitro)	H2O :~100 mg/mL (~106.61 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (106.61 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.0661 mL	5.3307 mL	10.6614 mL
	5 mM	0.2132 mL	1.0661 mL	2.1323 mL
	10 mM	0.1066 mL	0.5331 mL	1.0661 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.