Astragaloside I (Astrasieversianin IV; Cyclosieversioside B) is a natural product found in Astragalus membranaceus with osteogenic activity. It can stimulate osteoblast differentiation by modulating the Wnt/β-catenin signaling pathway.

Physicochemical Properties

Molecular Formula	C₄₅H₇₂O₁₆
Molecular Weight	869.04
CAS #	84680-75-1
PubChem CID	13996685
Appearance	White to off-white solid powder
Density	1.4±0.1 g/cm3
Boiling Point	910.6±65.0 °C at 760 mmHg
Flash Point	265.5±27.8 °C
Vapour Pressure	0.0±0.6 mmHg at 25°C
Index of Refraction	1.603
LogP	0.94
Hydrogen Bond Donor Count	7
Hydrogen Bond Acceptor Count	16
Rotatable Bond Count	11
Heavy Atom Count	61
Complexity	1690
Defined Atom Stereocenter Count	21
Synonyms	Cyclosieversioside B Astrasieversianin IV
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	In MC3T3-E1 cells, astragaloside I (10–40 μM) increases the expression of β-catenin, Runx2, BGP, and OPG, as well as RANKL (osteogenesis marker gene) [1].
Cell Assay	Western blot analysis[1] Cell Types: MC3T3-E1 cells. Tested Concentrations: 0, 10, 20, 40 μM. Incubation Duration: 5 days. Experimental Results: Stimulation of β-catenin and Runx2 expression. Cytotoxicity assay [1]. Cell Types: MC3T3-E1 cells. Tested Concentrations: 0, 10, 20, 40 μM. Incubation Duration: 1, 3 or 6 days (change medium every 2 days). Experimental Results: No obvious cytotoxic effect was found on MC3T3-E1 cells.
References	[1]. Astragaloside I Stimulates Osteoblast Differentiation Through the Wnt/β-catenin Signaling Pathway. Phytother Res. 2016 Oct;30(10):1680-1688.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~57.53 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 3.25 mg/mL (3.74 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 32.5 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 3.25 mg/mL (3.74 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 32.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.1507 mL	5.7535 mL	11.5070 mL
	5 mM	0.2301 mL	1.1507 mL	2.3014 mL
	10 mM	0.1151 mL	0.5753 mL	1.1507 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.