ATC-324 is an AR (Androgen Receptor) degrader based on the protein degradation technology platform AUTOphagy-TArgeting Chimera (AUTOTAC). ATC-324 induces the formation of AR/p62 complex, leading to autophagy-lysosomal degradation of AR. ATC-324 can reduce nuclear AR levels and downregulate target gene expression of AR and AR-v7, and also has a degradation effect on common AR mutants in PCa. ATC-324 is composed of target protein binding ligand (TBL) Enzalutamide and p62/SQSTM1 autophagy targeting ligand (ATL) YT 6-2 analog-1, connected by Boc-NH-PEG4-CH2CH2NH2 in the middle. Among them, the active control of the target protein ligand is Enzalutamide carboxylic acid, and the conjugate composed of the autophagy targeting ligand and linker is YT 6-2-PEG3-C2-NH2.

Physicochemical Properties

Molecular Formula	C53H55F6N5O10S
Molecular Weight	1068.09
CAS #	2925060-81-5
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. An Autophagy-Targeting Chimera Induces Degradation of Androgen Receptor Mutants and AR-v7 in Castration-Resistant Prostate Cancer. Cancer Res. 2024 Nov 12.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.9363 mL	4.6813 mL	9.3625 mL
	5 mM	0.1873 mL	0.9363 mL	1.8725 mL
	10 mM	0.0936 mL	0.4681 mL	0.9363 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.