6-Hydroxynicotinic acid is an endogenously produced metabolite.

Physicochemical Properties

Molecular Formula	C6H5NO3
Molecular Weight	139.11
Exact Mass	139.026
CAS #	5006-66-6
PubChem CID	72924
Appearance	White to off-white solid powder
Density	1.5±0.1 g/cm3
Boiling Point	488.8±30.0 °C at 760 mmHg
Melting Point	>300 °C(lit.)
Flash Point	249.4±24.6 °C
Vapour Pressure	0.0±1.3 mmHg at 25°C
Index of Refraction	1.626
LogP	-0.96
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	1
Heavy Atom Count	10
Complexity	239
Defined Atom Stereocenter Count	0
InChi Key	BLHCMGRVFXRYRN-UHFFFAOYSA-N
InChi Code	InChI=1S/C6H5NO3/c8-5-2-1-4(3-7-5)6(9)10/h1-3H,(H,7,8)(H,9,10)
Chemical Name	6-oxo-1H-pyridine-3-carboxylic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets

Microbial Metabolite Human Endogenous Metabolite

Additional Infomation

6-hydroxynicotinic acid is a monohydroxypyridine that is the 6-hydroxy derivative of nicotinic acid. It has a role as a mouse metabolite, a human urinary metabolite and an Arabidopsis thaliana metabolite. It is functionally related to a nicotinic acid. It is a conjugate acid of a 6-hydroxynicotinate(1-). 6-Hydroxynicotinic acid has been reported in Arabidopsis thaliana and Caenorhabditis elegans with data available.

Solubility Data

Solubility (In Vitro)

DMSO: 33.33 mg/mL (239.59 mM) H2O: 2 mg/mL (14.38 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.43 mg/mL (10.28 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.43 mg/mL (10.28 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 1.43 mg/mL (10.28 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 4: 1 mg/mL (7.19 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C).  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	7.1886 mL	35.9428 mL	71.8856 mL
	5 mM	1.4377 mL	7.1886 mL	14.3771 mL
	10 mM	0.7189 mL	3.5943 mL	7.1886 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.