4-Epidoxycycline is a liver metabolite of the antibiotic doxycycline and has no antibiotic function in mice. 4-Epidoxycycline has the same ability to regulate HER2 gene expression as doxycycline in in vitro experiments and in vivo mouse models, and also shows similar high efficiency as doxycycline in tumor tissues, achieving a tumor regression rate of more than 95%.

Physicochemical Properties

Molecular Formula	C22H24N2O8
Molecular Weight	444.43
CAS #	6543-77-7
SMILES	CC1C2C(C3C(C(=O)C(=C(C3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O
Synonyms	4-Epi-doxycycline
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. 4-Epidoxycycline: an alternative to doxycycline to control gene expression in conditional mouse models. Biochem Biophys Res Commun. 2004 Oct 22;323(3):979-86.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.2501 mL	11.2504 mL	22.5007 mL
	5 mM	0.4500 mL	2.2501 mL	4.5001 mL
	10 mM	0.2250 mL	1.1250 mL	2.2501 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.