2,3-Diaminonaphthalene is a selective colorimetric and fluorescent reagent that can be used for selenium detection and fluorometric determination of nitrite.

Physicochemical Properties

Molecular Formula	C10H10N2
Molecular Weight	158.1998
Exact Mass	158.084
CAS #	771-97-1
PubChem CID	69872
Appearance	Light yellow to brown solid powder
Density	1.2±0.1 g/cm3
Boiling Point	370.6±15.0 °C at 760 mmHg
Melting Point	197-203 °C
Flash Point	212.3±19.9 °C
Vapour Pressure	0.0±0.8 mmHg at 25°C
Index of Refraction	1.757
LogP	1.28
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	0
Heavy Atom Count	12
Complexity	140
Defined Atom Stereocenter Count	0
InChi Key	XTBLDMQMUSHDEN-UHFFFAOYSA-N
InChi Code	InChI=1S/C10H10N2/c11-9-5-7-3-1-2-4-8(7)6-10(9)12/h1-6H,11-12H2
Chemical Name	naphthalene-2,3-diamine
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

Poly(2,3-diaminonaphthalene) microspheres are being employed as novel quenchers for fluorescence-enhanced nucleic acid detection [1]. DAN, or 2,3-diaminonaphthalene, has the potential to cause cancer [2].

References

[1]. Poly(2,3-diaminonaphthalene) microspheres as a novel quencher for fluorescence-enhanced nucleic acid detection. Analyst. 2011 Jun 7;136(11):2221-4. [2]. Immobilization and characterization of 2,3-diaminonaphthalene/cyclodextrin complexes in a sol-gel matrix: a new fluorimetric sensor for nitrite. J Fluoresc. 2009 Jan;19(1):119-25. [3]. Effects of aminosalicylates and immunosuppressive agents on nitric oxide-dependent N-nitrosation reactions. Biochem Pharmacol. 1994 May 18;47(10):1897-902.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~632.11 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (15.80 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (15.80 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	6.3211 mL	31.6056 mL	63.2111 mL
	5 mM	1.2642 mL	6.3211 mL	12.6422 mL
	10 mM	0.6321 mL	3.1606 mL	6.3211 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.