| Description | DiR iodide (DiR iodide [1,1‘-dioctadecyl-3,3,3’,3‘-tetramethylindotricarbocyanine iodide]) is an important near infrared (NIR) fluorescent dye, which has been widely used for in vivo monitoring of cells. |
| In vitro | 染色贴壁细胞：在无菌玻璃盖玻片上培养贴壁细胞。从生长培养基中取出盖玻片，并轻轻去除多余的培养基。将盖玻片放入湿度箱中。向盖玻片的一角滴加100 μL染色工作液，并轻轻摇晃使所有细胞均被覆盖。在37℃下孵育盖玻片2-20分钟。去除染色工作液，并用预热的生长培养基清洗盖玻片两到三次。显微镜检测：使用DiI（549/565 nm，XF32-Omega，31002-Chroma）；DiR（748/780 nm，XF112-Omega，41009-Chroma）进行检测。 |
| Synonyms | DiR iodide [1,1‘-dioctadecyl-3,3,3’,3‘-tetramethylindotricarbocyanine iodide], DiR细胞膜荧光探针, DiR碘化物 |
| molecular weight | 1013.39 |
| Molecular formula | C63H101IN2 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| References | 1. Liu H, Wu D. In vivo Near-infrared Fluorescence Tumor Imaging Using DiR-loaded Nanocarriers. Curr Drug Deliv. 2016;13(1):40-8. |