| Description | 7,4'-Di-O-methylapigenin (4',7-DIMETHOXY-5-HYDROXYFLAVONE) can inhibit α-glucosidase and α-amylase enzymes and enhance 2-NBDG uptake in L6 cells, it has hypolipidemic effect on mouse pre-adipocyte (3T3L1) cell lines. |
| In vitro | Antioxidant activity in terms of TRP (196.03 μg/mg GAE), DPPH assay (IC50-173.09 μg/mL), hydroxyl radical scavenging assay (IC50-20.42 μg/mL), inhibiting LDL oxidation (IC50-51.99 μg/mL) and to enhance SOD activity (25.3 %) was higher in ethyl acetate extract (EAP). Phenolic and flavonoid content was measured and showed a positive correlation with antioxidant activity. Presence of 4',7-DIMETHOXY-5-HYDROXYFLAVONE and piperine (Pip) in EAP was determined by HPTLC analysis and was isolated. 4',7-DIMETHOXY-5-HYDROXYFLAVONE inhibited α-glucosidase and α-amylase enzymes and enhanced 2-NBDG uptake in L6 cells. Hypolipidemic effect of 4',7-DIMETHOXY-5-HYDROXYFLAVONE on mouse pre-adipocyte (3T3L1) cell lines also showed a dose dependent reduction on lipid droplet content and effective concentration range was determined as 1-2.5 μg/mL. Suggested that Piper longum fruits can provide a natural source of antioxidants with antidiabetic and anti obesity potential. |
| Cell experiments | Solvent extracts from Piper longum fruits using hexane, ethyl acetate, methanol, 70 % methanol-water were taken and 4',7-DIMETHOXY-5-HYDROXYFLAVONE was isolated from ethyl acetate extract. Antioxidant activity, glucose uptake potential and adipocyte differentiation assay was performed with extract and pure compound. |
| Synonyms | 芹菜素二甲醚, 4',7-DIMETHOXY-5-HYDROXYFLAVONE |
| molecular weight | 298.29 |
| Molecular formula | C17H14O5 |
| CAS | 5128-44-9 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 7 mg/mL (23.47 mM) |
| References | 1. Krishna M S , Joy B , Sundaresan A . Effect on oxidative stress, glucose uptake level and lipid droplet content by Apigenin 7, 4\"-dimethyl ether isolated fromPiper longumL[J]. Journal of Food Science and Technology, 2015, 52(6):3561-3570. |