| Description | 5-IAF is a fluorescein derivative of indoleacetamide, a fluorescent probe that can be used to label proteins and others containing free thiols (cysteine side chains). 5-IAF can label the catalytic (alpha) subunit of dog kidney Na,K-ATPase. |
| In vitro | 8 µM GSH 样品溶液分别与 8、80、200、400、800、1600、2000、2500 µM 5-IAF 进行衍生化处理。通过 CE-LIF 分析，其结果显示，使用 400 和 800 µM 5-IAF 时获得最高的信号强度。[2] |
| Synonyms | 5-Iodoacetamidofluorescein |
| molecular weight | 515.25 |
| Molecular formula | C22H14INO6 |
| CAS | 63368-54-7 |
| Storage | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMF: 90.0 mg/mL (174.7 mM ), Sonication and heating to 60℃ are recommended. DMSO: 45.0 mg/mL (87.3 mM), Sonication is recommended. |
| References | 1. Salvatore Sotgia, et al. Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection. PLoS One. 2013;8(7): e70374. 2. Yan Wang, et al. Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2009;1216(16):3533-3537. |