| Description | Monochlorobimane (mBBr) is a fluorescent dye with a λex value of 380 nm and a λem value of 470 nm.Monochlorobimane can be used to measure glutathione (GSH) in cells. |
| In vitro | 测量培养细胞中谷胱甘肽（GSH）的常用技术是向培养基中添加Monochlorobimane，其容易进入细胞并形成可通过荧光度量的GSH-Monochlorobimane加合物。Monochlorobimane方法与HPLC技术得到的结果具有可比性。Monochlorobimane浓度可降至25 mM，仍能准确测量均质物中的GSH含量。[1] |
| Synonyms | mBBr, Chlorobimane |
| molecular weight | 226.66 |
| Molecular formula | C10H11ClN2O2 |
| CAS | 76421-73-3 |
| Storage | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 45.0 mg/mL (198.5 mM), Sonication is recommended. |
| References | 1. Kamencic H, et al. Monochlorobimane fluorometric method to measure tissue glutathione. Anal Biochem. 2000 Nov 1;286(1):35-7. 2. Machado M D, et al. Assessment of cellular reduced glutathione content in Pseudokirchneriella subcapitata using monochlorobimane. Journal of applied phycology. 2012; 24: 1509-1516. 3. Hohwy M, et al. Novel prostaglandin D synthase inhibitors generated by fragment-based drug design. J Med Chem. 2008;51(7):2178-2186. |