| Description | 2',3'-cGAMP sodium (2'-3'-cyclic GMP-AMP sodium) is a second messenger in cellular innate immunity and is catalyzed by cGAMP synthase (cGAS) under DNA binding conditions. 2',3'-cGAMP sodium binds to STING to form a dimer, inducing the production and expression of interferon-β and other cytokines. |
| In vitro | 方法：WT C57BL/6J 小鼠的 BMDM 和 STINGgt 小鼠的 BMDM 用 2',3'-cGAMP sodium (20 µg/mL) 处理 24 h，检测细胞因子 IFNβ 水平。结果：2',3'-cGAMP 治疗显著刺激 WT C57BL/6J 小鼠 的 BMDM 产生 IFNβ，但在来自 STINGgt 小鼠的 BMDM 中没有。[1]方法：洋地黄素透化的 L929 细胞用 2',3'-cGAMP sodium (0-1000 nM) 处理 4 h，使用 qRT-PCR 检测 IFN-β RNA 水平。结果：2',3'-cGAMP 是细胞中 IFNβ 的有效诱导剂 (EC50:~20 nM)。[2] |
| In vivo | 方法：为研究对肥胖相关炎症和代谢疾病的作用，将 2',3'-cGAMP sodium (0.2 mg/kg) 腹腔注射给高脂肪饮食 (HFD) 或低脂肪饮食 (LFD) 的 C57BL/6 J 小鼠，每天一次，持续四周。结果：在肥胖小鼠中， 2',3'-cGAMP 治疗显著降低了饮食诱导的肝脏和脂肪组织的促炎反应，并改善了代谢失调。 2',3'-cGAMP 对巨噬细胞、肝细胞和脂肪细胞发挥细胞类型特异性抗炎作用。[1] |
| Synonyms | 2'-3'-cyclic GMP-AMP sodium |
| molecular weight | 718.37 |
| Molecular formula | C20H22N10Na2O13P2 |
| CAS | 2734858-36-5 |
| Storage | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: 45.0 mg/mL (62.6 mM), Sonication is recommended. DMSO: 0.9 mg/mL, Sonication is recommended. |
| References | 1. Guo X, et al. Cyclic GMP-AMP Ameliorates Diet-induced Metabolic Dysregulation and Regulates Proinflammatory Responses Distinctly from STING Activation. Sci Rep. 2017 Jul 25;7(1):6355. 2. Zhang X, et al. Cyclic GMP-AMP containing mixed phosphodiester linkages is an endogenous high-affinity ligand for STING. Mol Cell. 2013 Jul 25;51(2):226-35. 3. Su M, et al. Second messenger 2'3'-cyclic GMP-AMP (2'3'-cGAMP): Synthesis, transmission, and degradation. Biochem Pharmacol. 2022;198:114934. 4. Lai J, et al. Zebularine elevates STING expression and enhances cGAMP cancer immunotherapy in mice. Mol Ther. 2021;29(5):1758-1771. |