| Description | Vincristine is an alkaloidal natural product that binds to microtubule proteins and inhibits the formation of microtubules, thereby inhibiting mitosis in tumor cells. Vincristine is used as a microtubule destabilizing agent in research studies for the treatment of hematologic neoplasms such as leukemias and lymphomas, as well as in studies related to sarcomas in children. |
| In vitro | 方法：神经母细胞瘤细胞 SH-SY5Y 用 Vincristine (0.001-10 µM) 处理 24-72 h，使用 MTT assay 检测细胞活力。结果：Vincristine 对 SH-SY5Y 细胞增殖的抑制作用具有剂量和时间依赖性。24、48 和 72 h的 IC50 分别为0.113 µM、0.078 µM 和 0.051 µM。[1]方法：人白血病细胞 MOLT-4 用 Vincristine (0.3-3 µM) 和 SAHA (500 nM) 处理 24-48 h，使用 Flow cytometry 检测细胞周期。结果：与 SAHA 相比，Vincristine 处理诱导细胞周期的 G2/M 期增加。Vincristine 加 SAHA 的组合在短期治疗后 (24 h) 导致细胞几乎完全停滞在 G2/M 期，随后在长期治疗后 (48 h) 诱导细胞进入 sub-G1 期。[2] |
| In vivo | 方法：为检测体内抗肿瘤活性，将 Vincristine (0.025 mg/kg，静脉注射，每周一次) 和 SAHA (200 mg/kg，口服给药，每天一次) 给药给携带 MOLT-4 异种移植物的 SCID 小鼠，持续 24 天。结果：单独用 Vincristine 或 SAHA 治疗的小鼠的 TGD 没有改善。然而，对数秩分析显示，在 MOLT-4 异种移植物模型中，共处理表现出显著的抗肿瘤活性。[2] |
| molecular weight | 824.96 |
| Molecular formula | C46H56N4O10 |
| CAS | 57-22-7 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 12 mg/mL (14.55 mM) |
| References | 1. Tu Y, et al. Vincristine induces cell cycle arrest and apoptosis in SH-SY5Y human neuroblastoma cells. Int J Mol Med. 2013 Jan;31(1):113-9. 2. Chao MW, et al. The synergic effect of vincristine and vorinostat in leukemia in vitro and in vivo. J Hematol Oncol. 2015 Jul 10;8:82. |