| Description | Ribonuclease A (RNase A) is a potent endonuclease that removes RNA during dna and protein preparation. |
| In vitro | RNase A 主要用于去除质粒DNA或基因组DNA制备过程中的RNA。制备10 mg/mL RNase A储备液时，首先使用10 mM醋酸钠溶液（pH 5.2）进行配制；随后将溶液在100℃下加热15分钟；冷却至室温后，加入体积的十分之一的1 M Tris-HCl溶液（pH 7.4），并调整pH至7.4。最后，将储备液在-20℃条件下保存，其稳定性可达2年。注意：RNase A溶液在中性条件下沸腾可导致沉淀形成，而在较低pH条件下沸腾若出现沉淀，则可能是由蛋白杂质引起，该沉淀可通过13,000 rpm的高速离心除去。 |
| Synonyms | EC 3.1.27.5, RNase A |
| molecular weight | N/A |
| CAS | 9001-99-4 |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: 80 mg/mL, Sonication is recommended. |
| References | 1. Thakur P, et al. Locating chemical modifications in RNA sequences through ribonucleases and LC-MS based analysis. Methods Enzymol. 2021;658:1-24. 2. Wallace JA, et al. Predicting pKa values with continuous constant pH molecular dynamics. Methods Enzymol. 2009;466:455-75. 3. Brown D, et al. Ribonuclease-resistant RNA controls and standards. Methods Enzymol. 2001;341:648-54. |