p-Nitrophenyl α-L-arabinopyranoside_product_DataBase

Bioactivity	p-Nitrophenyl α-L-arabinopyranoside is a biochemical reagent. p-Nitrophenyl α-L-arabinopyranoside can be hydrolyzed by recombinant BgaA (rBgaA, isolated from E. coli BL21 (DE3) strain harboring pEBGA29). p-Nitrophenyl α-L-arabinopyranoside has potential application in enzyme activity detection[1][3].
Invitro	p-Nitrophenyl α-L-arabinopyranoside 与 rBgaA 的亲和度 Km 值为 6.06 mM[1]。p-Nitrophenyl α-L-arabinopyranoside 对 xylosidase–arabinosidase (xarB) 基因具有高活性[2]。p-Nitrophenyl α-L-arabinopyranoside 在酶活性检测中的应用[3](1) 200 µL 2 mM p-Nitrophenyl α-L-arabinopyranoside、100 µL 酶、300 µL 50 mM 磷酸盐缓冲液 (pH 7.0)，在 37℃ 下共孵育 0.5、1 和 5 h。(2) 加入 400 µL 0.5 M 氢氧化钠，终止反应。(3) 在 405 nm 处测量混合物的吸光度。注：在人参皂苷存在的情况下，用丁醇萃取终止反应。
Name	p-Nitrophenyl α-L-arabinopyranoside
CAS	1223-07-0
Formula	C11H13NO7
Molar Mass	271.22
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Kosugi A, et al. Characterization of two noncellulosomal subunits, ArfA and BgaA, from Clostridium cellulovorans that cooperate with the cellulosome in plant cell wall degradation. J Bacteriol. 2002 Dec;184(24):6859-65. [2]. Mai V, et al. Cloning, sequencing, and characterization of the bifunctional xylosidase-arabinosidase from the anaerobic thermophile thermoanaerobacter ethanolicus. Gene. 2000 Apr 18;247(1-2):137-43. [3]. Shin H Y, et al. Purification and Characterization ofα-l-Arabinopyranosidase andα-l-Arabinofuranosidase from Bifidobacterium breve K-110, a Human Intestinal Anaerobic Bacterium Metabolizing Ginsenoside Rb2 and Rc[J]. Applied and environmental microbiology, 2003, 69(12): 7116-7123.

PeptideDB

p-Nitrophenyl α-L-arabinopyranoside

CAS: 1223-07-0 F: C11H13NO7 W: 271.22