| Bioactivity | Yoda 1 is a potent and selective Piezo1 agonist. Yoda 1 activates purified Piezo1 channels. Yoda 1 potently inhibits macropinocytosis induced by epidermal growth factor (EGF). Yoda 1 enhances Ca2+ influx followed by activation of the calcium-activated potassium channel KCa3.1 and inhibition of Rac1 activation[1][2][3]. | ||||||||||||
| Invitro | Yoda1 (0-6 μM, 5 min) induces the activation of both Akt and ERK1/2, which is not dependent on Piezo1[2].Yoda1 (1.5 μM, 5 min) inhibits Rac1 activation[3]. Western Blot Analysis Cell Line: | ||||||||||||
| Name | Yoda 1 | ||||||||||||
| CAS | 448947-81-7 | ||||||||||||
| Formula | C13H8Cl2N4S2 | ||||||||||||
| Molar Mass | 355.27 | ||||||||||||
| Appearance | Solid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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| Reference | [1]. Syeda R, et al. Chemical activation of the mechanotransduction channel Piezo1. Elife. 2015 May 22;4. [2]. Dela Paz NG, et al. Yoda1-induced phosphorylation of Akt and ERK1/2 does not require Piezo1 activation. Biochem Biophys Res Commun. 2018 Feb 26;497(1):220-225. [3]. Kuriyama M, et al. Piezo1 activation using Yoda1 inhibits macropinocytosis in A431 human epidermoid carcinoma cells. Sci Rep. 2022 Apr 15;12(1):6322. |