| Bioactivity | XY221 (Compound 16o) selectively inhibits BRD4 BD2, with an IC50 of 5.8 nM. XY221 demonstrates high pan-BD2 selectivity (667-fold over BRD4 BD1) and BRD4 BD2 domain selectivity (9−32-fold over BRD2/3/T BD2). XY221 induce Apoptosis in MV4-11 cells and shows anticancer activity [1]. |
| Invitro | XY221 抑制 BRD4 BD2, BRD3 BD2, BRD2 BD2, BRDT BD2 and BRD4 BD1 的 IC50 值分别为 5.8, 70.98, 53.47, 183, 3869 nM[1]。XY221 抑制 MV4−11, LNCaP, HL-7702 cells 增殖的 IC50 分别为 0.51, 0.68, 47.7 μM[1]。XY221 (500-2000 nM，72 h) 能显著抑制 MV4-11 细胞的增殖和诱导 MV4-11 细胞凋亡[1]。XY221 (1-10 μM，2 h) 在 RLM (鼠肝线粒体) 中半衰期长达 120 min 以上，在 HLM (人肝线粒体) 中半衰期也长达 120 min 以上[1]。XY221 (500-2000 nM, 72h) 抑制 MV4−11 细胞中靶基因 p21 ,ODC1, BRD4 和 AKT 、AMPK 的磷酸化[1]。XY221 (2 μM, 72h) 在 mRNA 水平上抑制 MV4−11 细胞原癌基因 MYC 及其靶基因 p21 和细胞周期相关基因 BCL-2[1]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> XY221 相关抗体: Western Blot Analysis[1] Cell Line: |
| In Vivo | XY221 在大鼠中静脉 (iv) 和口服给药(po) 时的药代动力学参数 route |
| Formula | C32H34FN3O5 |
| Molar Mass | 559.63 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Tong JB, et al. Discovery of novel BRD4-BD2 inhibitors via in silico approaches: QSAR techniques, molecular docking, and molecular dynamics simulations. Mol Divers. 2024 Apr;28(2):671-692. |