| Bioactivity | X-Gluc sodium is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator). X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue)[1]. |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).1. Dissolve 20 mg X-Gluc sodium in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix.2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization.3. Allow the plates to air dry and be used to inoculate the organisms.4. Incubate the plates at 35°C and observe 16-24 h after inoculation. |
| Name | X-Gluc sodium |
| CAS | 129541-41-9 |
| Formula | C14H12BrClNNaO7 |
| Molar Mass | 444.59 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| Reference | [1]. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404. |
X-Gluc sodium
CAS: 129541-41-9 F: C14H12BrClNNaO7 W: 444.59
X-Gluc sodium is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc sodium
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