Thiazolyl Blue_product_DataBase

Bioactivity	Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis[1]. Storage: protect from light.
Invitro	1. 制备储存液用 PBS 配制 5 mg/mL 储备液，用 0.22 μm 滤膜过滤除菌，4℃ 避光短时间保存。2.细胞增值检测 (以96孔板为例)2.1 接种细胞：用含 10％胎牛血清的培养液配成单个细胞悬液，以每孔 1000-10000 个细胞接种到 96 孔板，每孔体积 100 uL。2.2 培养细胞：37℃，5% CO2，培养 24-72 h。2.3 每孔加入10 μL MTT 溶液 (5 mg/mL)，孵育 4 小时，终止培养。小心吸去孔内培养上清液，对于悬浮细胞需离心后再吸去孔内上清液。2.4 每孔加入100 uL DMSO，置摇床上低速振荡 10 分钟，使结晶物充分溶解。2.5 比色：选择 490 nm波长，在酶联免疫监测仪上测定各孔光吸收值，记录结果，以时间为横坐标，吸光值为纵坐标绘制细胞生长曲线。
Name	Thiazolyl Blue
CAS	298-93-1
Formula	C18H16BrN5S
Molar Mass	414.32
Appearance	Solid
Transport	Room temperature in continental US; may vary elsewhere.
Storage	4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Reference	[1]. Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52. [2]. Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.

PeptideDB

Thiazolyl Blue

CAS: 298-93-1 F: C18H16BrN5S W: 414.32