| Bioactivity | TRFS-red, a red fluorescence emission off-on probe, is selective for thioredoxin reductase (TrxR). TRFS-red exhibits high response rate and sensitivity. TRFS-red can be used for imaging live cells[1]. |
| Invitro | TRFS-red (1 μM; 30-120 min) gives red fluorescence signal in live HeLa cells, and the red fluorescence is predominantly distributed in the cytosol[1].TRFS-red (10 μM) has a maximal absorption at ~530 nm in TE buffer (50 mM Tris-HCl and 1 mM EDTA, pH 7.4)[1].TRFS-red displays a reliable fluorescence signal in the pH range of 5.5-8.5[1]. |
| Name | TRFS-red |
| Formula | C28H28ClN3O11S2 |
| Molar Mass | 682.12 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| Reference | [1]. Ma H, et, al. A fast response and red emission probe for mammalian thioredoxin reductase. Chem Commun (Camb). 2016 Oct 4;52(81):12060-12063. |