| Bioactivity | Sudan Black B, a fat-soluble diazo dye, is a histochemical stain. Sudan Black B can be used for staining of neutral triglycerides and lipids[1]. | ||||||||||||
| Invitro | Sudan Black B works also in frozen material, and therefore can be complementarily used with SA-β-gal when investigating senescence. Sudan Black B is a highly lipophilic agent that exhibits high affi nity to the lipid compartment of lipofuscin. The positive Sudan Black B-lipofuscin reaction reveals blue-black intracellular granules in cells and frozen tissues and brown to black granules in FFPE tissues under the light microscope. | ||||||||||||
| Name | Sudan Black B | ||||||||||||
| CAS | 4197-25-5 | ||||||||||||
| Formula | C29H24N6 | ||||||||||||
| Molar Mass | 456.54 | ||||||||||||
| Appearance | Solid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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| Reference | [1]. Konstantinos Evangelou, et al. Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells. Methods Mol Biol. 2017;1534:111-119. |