PeptideDB

Suc-Leu-Leu-Val-Tyr-AMC

CAS: 94367-21-2 F: C40H53N5O10 W: 763.88

Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate.
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Bioactivity Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate.
Invitro Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY) is a membrane-permeable calpain-specific fluorogenic substrate, pteolytic hydrolysis of the peptidyl-7-amino bond liberates the highly fluorescent 7-amino-4-methylcoumarin (AMC) moiety[1]. The effectof TGF-β on hydrolysis of these substrates (e.g Suc-Leu-Leu-Val-Tyr-AMC) are assessed. Biliary epithelial H69 cells are incubated with 10, 1, 0.1, or 0 ng/mL TGF-β for 24 h. Substrate hydrolysis is then fluorometrically assessed in cytosolic extracts. Basal activity is 1.12, 8.33, and 14.52 nmol AMC/mg protein/min for suc-LLVY-AMC, z-LLE-AMC, and z-LLL-AMC hydrolysis, respectively[2].
Name Suc-Leu-Leu-Val-Tyr-AMC
CAS 94367-21-2
Sequence Suc-Leu-Leu-Val-Tyr
Shortening Suc-LLVY
Formula C40H53N5O10
Molar Mass 763.88
Transport Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light, under nitrogen

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)