| Bioactivity | Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate. | ||||||
| Invitro | Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY) is a membrane-permeable calpain-specific fluorogenic substrate, pteolytic hydrolysis of the peptidyl-7-amino bond liberates the highly fluorescent 7-amino-4-methylcoumarin (AMC) moiety[1]. The effectof TGF-β on hydrolysis of these substrates (e.g Suc-Leu-Leu-Val-Tyr-AMC) are assessed. Biliary epithelial H69 cells are incubated with 10, 1, 0.1, or 0 ng/mL TGF-β for 24 h. Substrate hydrolysis is then fluorometrically assessed in cytosolic extracts. Basal activity is 1.12, 8.33, and 14.52 nmol AMC/mg protein/min for suc-LLVY-AMC, z-LLE-AMC, and z-LLL-AMC hydrolysis, respectively[2]. | ||||||
| Name | Suc-Leu-Leu-Val-Tyr-AMC | ||||||
| CAS | 94367-21-2 | ||||||
| Sequence | Suc-Leu-Leu-Val-Tyr | ||||||
| Shortening | Suc-LLVY | ||||||
| Formula | C40H53N5O10 | ||||||
| Molar Mass | 763.88 | ||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||
| Storage | Sealed storage, away from moisture and light, under nitrogen
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen) |