Stains-All_product_DataBase

Bioactivity	Stains-All, a cationic carbocyanine dye, is a convenient probe to study the structural features of the individual calcium-binding sites of calmodulin (CaM) and related calcium-binding proteins (CaBP)[1][2].
Invitro	Almost all of the proteins found in skeletal muscle extracts, including the Ca2++Mg2+-ATPase and the 53,000-Da glycoprotein of the sarcoplasmic reticulum, are stained red or pink with Stains-all. Calsequestrin, the 1 60,000-Da glycoprotein, and 170,000-Da protein are stained blue with Stains-all. The ratio of Stains-all staining (measured at 615 nm) to that of Coomassie blue staining (measured at 575 nm) is 1.3 for calsequestrin, 2.0 for calmodulin, 1.4 for troponin C, and 2.2 for S-100. Therefore, in addition to differentially staining these Ca2+-binding proteins blue, Stains-all is a more sensitive stain for these Ca2+-binding proteins than is Coomassie blue[3].
Name	Stains-All
CAS	7423-31-6
Formula	C30H27BrN2S2
Molar Mass	559.58
Appearance	Solid
Transport	Room temperature in continental US; may vary elsewhere.
Storage	4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Reference	[1]. Gruber HE, et al. Application of stains-all for demarcation of cement lines in methacrylate embedded bone. Biotech Histochem. 1991;66(4):181-184. [2]. Sharma Y, et al. Studies on the interaction of the dye, stains-all, with individual calcium-binding domains of calmodulin. FEBS Lett. 1993;326(1-3):59-64. [3]. Campbell KP, et al. Staining of the Ca2+-binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye "Stains-all". J Biol Chem. 1983;258(18):11267-11273.

PeptideDB

Stains-All

CAS: 7423-31-6 F: C30H27BrN2S2 W: 559.58