| Bioactivity | Sphingosine-1-phosphate (S1P) is an agonist of S1P1-5 receptors and a ligand of GPR3, GPR6 and GPR12. Sphingosine-1-phosphate is an intracellular second messenger and mobilizes Ca2+ as an extracellular ligand for G protein-coupled receptors[1]. Sphingosine-1-phosphate is an important lipid mediator generated from Sphingomyelin (HY-113498) or other membrane phospholipids[2]. |
| Invitro | S1P (1 μM) induces a significant Ca2+ releases in HEK293 cells under serum starvation conditions (1% FCS)[1].In a functional Ca2+ assay, Suramin (HY-B0879) alone does not exert any effect on intracellular Ca2+ release via gpr3, gpr6 or gpr12. In contrast, S1P (1 μM) induces Ca2+ release of gpr3, gpr6 and gpr12 in the presence of Suramin (HY-B0879) various concentrations in transfected HEK293 cells[2]. In a functional Ca2+ assay,S1P (3-3000 nM) in the presence Suramin (300 μM), exhibits nanomolar EC50 values for gpr3 (EC50=29 nM), gpr6 (EC50=15 nM) and gpr12 (EC50=24 nM), rat gpr3 (EC50=68 nM),respectively in HEK293 cells[2]. S1P increases intracellular calcium levels in TAg-Jurkat cells expressing S1P1 and Gqi5, which allows for phospholipase C stimulation by Gi proteins, when used at a concentration of 200 nM, as well as in TAg-Jurkat cells expressing S1P2 and S1P3 receptors (EC50s = 8 and 11 nM, respectively)[3]. |
| Name | Sphingosine-1-phosphate |
| CAS | 26993-30-6 |
| Formula | C18H38NO5P |
| Molar Mass | 379.47 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | -20°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
Sphingosine-1-phosphate
CAS: 26993-30-6 F: C18H38NO5P W: 379.47
Sphingosine-1-phosphate (S1P) is an agonist of S1P1-5 receptors and a ligand of GPR3, GPR6 and GPR12. Sphingosine-1-pho
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