Spectinomycin dihydrochloride pentahydrate_product_DataBase

Bioactivity	Spectinomycin dihydrochloride pentahydrate is a broad-spectrum antibiotic and inhibits the growth of a variety of gram-positive and gram-negative organisms. Spectinomycin dihydrochloride pentahydrate acts by selectively targeting to the bacterial ribosome and interrupting protein synthesis. Spectinomycin dihydrochloride pentahydrate is also a noncompetitive inhibitor of td intron RNA with an Ki value of 7.2 mM[1]-[5].
Invitro	Spectinomycin dihydrochloride pentahydrate selectively inhibits protein synthesis in cells and in extracts of Escherichia coli: Spectinomycin dihydrochloride pentahydrate (50 μg/mL) inhibits Escherichia coli growth rapidly and reversibly, and suppresses amino acid incorporation immediately[1]. Spectinomycin dihydrochloride pentahydrate (1 μg/mL or 3 μM) inhibits polypeptide synthesis directed either by endogenous messenger RNA or by MS-2 bacteriophage RNA, with maximum inhibition of 70-80% in extracts of Escherichia coli[1]. Spectinomycin dihydrochloride pentahydrate blocks the translocation of peptidyl-tRNAs from A-site to P-site by inhibiting the binding of elongation factor G to the ribosome[2]. Spectinomycin dihydrochloride pentahydrate interacts specifically with the residues G1064 and 01192 in 16S rRNA and potentially makes it inactive[2].Spectinomycin dihydrochloride pentahydrate exhibits splicing inhibition and dependent on pH changes and Mg2+ concentration, indicating electrostatic interactions with the intron RNA[3].
Name	Spectinomycin dihydrochloride pentahydrate
CAS	22189-32-8
Formula	C14H36Cl2N2O12
Molar Mass	495.35
Transport	Room temperature in continental US; may vary elsewhere.
Storage	4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

PeptideDB

Spectinomycin dihydrochloride pentahydrate

CAS: 22189-32-8 F: C14H36Cl2N2O12 W: 495.35