Rhod-2 AM_product_DataBase

Bioactivity	Rhod-2 is a high-affinity visible light excitation wavelength Ca2+ fluorescent probe, Rhod-2, AM is an acetyl methyl ester derivative of Rhod-2, which has cell membrane permeability and can easily enter cells with simple culture. Once it enters the cell, it is sheared by its lactesterase to produce Rhod-2 without membrane permeability, which remains in the cell to perform the corresponding physiological functions. Maximum excitation/emission wavelength: 549/578 nm[1]. Storage: protect from light.
Invitro	1.Rhod-2 AM 工作液的配制1.1 制备储存液用无水DMSO 配制 5 mM 的 Rhod-2 AM。1.2 工作液的配制用预热好的无血清细胞培养基或 PBS 稀释储存液，配制成 1-10 μM 的 Rhod-2 AM 工作液。注：请根据实际情况调整 Rhod-2 AM 工作液浓度，且现用现配。2. 细胞染色(悬浮细胞)2.1 悬浮细胞：在 4℃ 下 1000 g 离心 3-5 分钟，然后丢弃上清液。加入 PBS 洗涤两次，每次 5 分钟。细胞密度在 1×106/mL。2.2 加入 1 mL Rhod-2 AM 工作液，室温孵育 5-30 分钟。2.3 400 g，离心 3-4 分钟，弃去上清。2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，使用荧光显微镜或流式细胞仪进行观察。3. 细胞染色(贴壁细胞)3.1 将贴壁细胞培养于无菌盖玻片上。3.2 从培养基中移出盖玻片，吸除多余培养基。3.3 加入 100 μL 染料工作液，轻轻晃动使其完全覆盖细胞，室温孵育 5-30 分钟。3.4 吸去染料工作液，用培养基洗 2-3 次，每次 5 分钟，使用荧光显微镜或流式细胞仪进行观察。
Name	Rhod-2 AM
CAS	145037-81-6
Formula	C52H59BrN4O19
Molar Mass	1123.94
Appearance	Oil
Transport	Room temperature in continental US; may vary elsewhere.
Storage	-20°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Reference	[1]. Brisac C, et al. Calcium flux between the endoplasmic reticulum and mitochondrion contributes to poliovirus-induced apoptosis. J Virol. 2010 Dec;84(23):12226-35. [2]. Brisac C, et al. Calcium flux between the endoplasmic reticulum and mitochondrion contributes to poliovirus-induced apoptosis. J Virol. 2010 Dec;84(23):12226-35.

PeptideDB

Rhod-2 AM

CAS: 145037-81-6 F: C52H59BrN4O19 W: 1123.94