| Bioactivity | RhoNox-1 is a fluorescent probe for the specific detection of divalent iron ions, and when RhoNox-1 reacts with Fe2+. RhoNox-1 can generate an irreversible orange (red) fluorescent product (Ex/Em：540/575 nm) . FeRhoNox-1 can enter the cell well, suitable for the detection of Fe2+ in living cells, and tends to be localized in the Golgi apparatus[1]. | ||||||||||||
| Invitro | 1.RhoNox-1 工作液的配制1.1 制备储存液用 110 μL 无水 DMSO 配制 50 μg 的 RhoNox-1 得到 1 mM 储备液。1.2 工作液的配制用预热好的无血清细胞培养基或 PBS 稀释储存液，配制成 1-10 μM 的 RhoNox-1 工作液。注：请根据实际情况调整 RhoNox-1 工作液浓度，且现用现配。2. 细胞染色(悬浮细胞)2.1 悬浮细胞：离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。细胞密度在1×106/mL2.2 加入 1 mL工作液，室温孵育 5-30 分钟。2.3 400 g，离心 3-4 分钟，弃去上清。2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，使用荧光显微镜或流式细胞仪进行观察。3. 细胞染色(贴壁细胞)3.1 将贴壁细胞培养于无菌盖玻片上。3.2 从培养基中移出盖玻片，吸除多余培养基。3.3 加入 100μL 染料工作液，轻轻晃动使其完全覆盖细胞，室温孵育5-30 分钟。3.4 吸去染料工作液，用培养基洗 2-3 次，每次 5 分钟 ，使用荧光显微镜或流式细胞仪进行观察。 | ||||||||||||
| Name | RhoNox-1 | ||||||||||||
| CAS | 1447815-38-4 | ||||||||||||
| Formula | C28H30N2O4 | ||||||||||||
| Molar Mass | 458.55 | ||||||||||||
| Appearance | Solid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
|
||||||||||||
| Reference | [1]. Mukaide T, et al. Histological detection of catalytic ferrous iron with the selective turn-on fluorescent probe RhoNox-1 in a Fenton reaction-based rat renal carcinogenesis model. Free Radic Res. 2014 Sep;48(9):990-5. [2]. Jamnongkan W, et al. Upregulation of transferrin receptor-1 induces cholangiocarcinoma progression via induction of labile iron pool. Tumour Biol. 2017 Jul;39(7):1010428317717655. [3]. Ito F, et al. Contrasting intra- and extracellular distribution of catalytic ferrous iron in ovalbumin-induced peritonitis. Biochem Biophys Res Commun. 2016 Aug 5;476(4):600-606. |
RhoNox-1
CAS: 1447815-38-4 F: C28H30N2O4 W: 458.55
RhoNox-1 is a fluorescent probe for the specific detection of divalent iron ions, and when RhoNox-1 reacts with Fe2+. Rh
Sales Email：peptidedb@qq.com
This product is for research use only, not for human use. We do not sell to patients.