Bioactivity | Resorufin butyrate is a fluorogenic substrate for triglyceride lipases and cholinesterase (Ex=570 nm, Em=580 nm)[1][2][3]. Storage: protect from light. |
Target | Triglyceride lipases, cholinesterase |
Invitro | Resorufin butyrate (2.3 μM) displays a linear increase in fluorescence over 60 min (conducted in 384-well plates using 12 nM of bLPL enzyme and substrate in PBS, pH 7.5, 5% DMSO)[1]. |
Name | Resorufin butyrate |
CAS | 15585-42-9 |
Formula | C16H13NO4 |
Molar Mass | 283.28 |
Transport | Room temperature in continental US; may vary elsewhere. |
Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
Reference | [1]. J Biomol Screen, et al. Resorufin butyrate as a soluble and monomeric high-throughput substrate for a triglyceride lipase. J Biomol Screen. 2012 Feb;17(2):245-51. [2]. Guilbault, G.G., et al. Resorufin butyrate and indoxyl acetate as fluorogenic substrates for chloinesterase. Anal Chem. 1965 Jan;37:120-3. [3]. J.Hofmann, et al. Immobilized enzyme kinetics analyzed by flow-through microfluorimetry : Resorufin-β-d-galactopyranoside as a New Fluorogenic Substrate for β-Galactosidase. Anal. Chim. Acta 163, 67-72 (1984). |