| Bioactivity | RFRP-3 (mouse) is a functional ortholog of avian gonadotropin inhibitory hormone (GnIH), binding to GPR147. RFRP-3 (mouse) reduces Progesterone synthesis by inhibiting FSHR and key enzymes involved in steroidogenesis (P450scc, 3β-HSD, StAR). RFRP-3 (mouse) induces Apoptosis (increase of p53). RFRP-3 (mouse) also suppresses the ERK signaling pathway. RFRP-3 (mouse) can be used for research of follicular development[1]. |
| Invitro | RFRP-3 (10-1000 ng/mL, 24 h) 能显著减少小鼠卵巢颗粒细胞中 Progesterone (HY-N0437) 的分泌，但不能减少 Estradiol (HY-B0141) 的分泌[1]。RFRP-3 (10-1000 ng/mL, 24 h) 抑制小鼠卵巢颗粒细胞中类固醇生成和 FSHR (抑制 p450scc、3β-HSD、StAR 和 FSHR)[1]。RFRP-3 (10-1000 ng/mL, 24 h) 诱导小鼠卵巢颗粒细胞凋亡，在中等剂量下降低 Bcl-2 蛋白表达，增加 p53 蛋白表达[1]。RFRP-3 (10-1000 ng/mL, 24 h) 抑制小鼠卵巢颗粒细胞中 ERK 信号通路 (p-ERK1/2 蛋白)[1]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> RFRP-3 (mouse) 相关抗体: Real Time qPCR[1] Cell Line: |
| Sequence | Val-Asn-Met-Glu-Ala-Gly-Thr-Arg-Ser-His-Phe-Pro-Ser-Leu-Pro-Gln-Arg-Phe-NH2 |
| Shortening | VNMEAGTRSHFPSLPQRF-NH2 |
| Formula | C91H141N29O25S |
| Molar Mass | 2073.34 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Guo X, et al. Rfamide-related peptide-3(RFRP-3) receptor gene is expressed in mouse ovarian granulosa cells: Potential role of RFRP-3 in steroidogenesis and apoptosis. Steroids. 2024 Feb;202:109349. |