Phalloidin-TRITC_product_DataBase

Bioactivity

Phalloidin-TRITC is a fluorescein derivative of Phalloidin, which can specifically label myof lin and display red fluorescence when labeled and can be observed using Tesred channels[1].

Invitro

1.1 制备储存液用 DMSO 配制 1mM 储存液。1.2 工作液的配制用预热好的无血清细胞培养基或 PBS 稀释储存液，终浓度为 200-1000 nM。注：请根据实际情况调整 Phalloidin-TRITC 工作液浓度，且现用现配。2.细胞染色2.1 悬浮细胞：离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。贴壁细胞：弃去培养基，加入胰蛋白酶消化细胞。离心弃去上清后，加入 PBS 洗涤两次，每次 5 分钟。2.2 加入 1 mL Phalloidin-TRITC 工作液，室温孵育 5-10 分钟。2.3 400 g，4℃ 离心 3-4 分钟，弃去上清。2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，使用荧光显微镜进行观察。

Name

Phalloidin-TRITC

CAS

915013-10-4

Shortening

A-{d-Thr}-C-{Hyp}-AW-{Thr} (Covalent bridge:Cys3-Trp6)

Formula

C60H70N12O13S2

Molar Mass

1231.40

Appearance

Solid

Transport

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Reference

[1]. J A Cooper, et al. Effects of cytochalasin and phalloidin on actin. J Cell Biol. 1987 Oct;105(4):1473-8. [2]. J A Cooper, et al. Effects of cytochalasin and phalloidin on actin. J Cell Biol. 1987 Oct;105(4):1473-8. [3]. J Wehland, et al. Phalloidin-induced actin polymerization in the cytoplasm of cultured cells interferes with cell locomotion and growth. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5613-7.

PeptideDB

Phalloidin-TRITC

CAS: 915013-10-4 F: C60H70N12O13S2 W: 1231.40