| Bioactivity | PFM01, N-alkylated Mirin derivative, is a MRE11 endonuclease inhibitor. PFM01 can regulate double-strand break repair (DSBR) by nonhomologous end-joining (NHEJ) versus homologous recombination (HR)[1][2]. | |||||||||
| Target | Endonuclease | |||||||||
| Invitro | PFM01 (100 μM) rescues the repair defect in 48BR (WT) and HSC62 (BRCA2-defective) primary cells[1].PFM01 (100 μM) diminishes the RAD51 foci formation in 1BR3 (WT) and HSC62 (BRCA2-defective) cells[1].PFM01 (100 μM) enhances non-homologous end-joining (NHEJ) in H1299 dA3 cells and reduces homologous recombination (HR) in U2OS DR-GFP cells[1].PFM01 substantially relieves the double-strand break (DSB) repair defect confers by mirin or PFM39 in irradiated G2 cells[1]. | |||||||||
| Name | PFM01 | |||||||||
| CAS | 1558598-41-6 | |||||||||
| Formula | C14H15NO2S2 | |||||||||
| Molar Mass | 293.40 | |||||||||
| Appearance | Solid | |||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | |||||||||
| Storage |
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| Reference | [1]. Shibata A, et, al. DNA double-strand break repair pathway choice is directed by distinct MRE11 nuclease activities. Mol Cell. 2014 Jan 9; 53(1): 7-18. [2]. Völkening L, et, al. RAD50 regulates mitotic progression independent of DNA repair functions. FASEB J. 2020 Feb; 34(2): 2812-2820. |