| Bioactivity | PARL-IN-1 is a potent PARL inhibitor with an IC50 value of 28 nM. PARL-IN-1 inhibits PARL and leads to a robust activation of the PINK1/Parkin pathway. PARL-IN-1 promotes PINK1/Parkin-dependent mitophagy[1]. |
| Target | IC50: 28 nM (PARL) |
| Invitro | PARL-IN-1 (compound 5; 5 nM-20 μM) 以剂量依赖性方式阻止细胞中线粒体应激诱导的 PARL 对 PGAM 的切割，并抑制 HEK293T 细胞中过表达的 PGAM5 的切割[1] 。PARL-IN-1 (0.1-30 μM; 8 h; HEK293T 细胞) 稳定 PINK1 并触发其在活细胞中的选择性切割和运输[1]。PARL-IN-1 (5 μM; 22 h; HEK293 T-REx 细胞) 阻断导致异常活性氧 (ROS) 产生的呼吸链，激活 PINK1/Parkin 通路[1]。 Western Blot Analysis[1] Cell Line: |
| Name | PARL-IN-1 |
| Formula | C40H58N6O7 |
| Molar Mass | 734.92 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Poláchová E, et, al. Chemical Blockage of the Mitochondrial Rhomboid Protease PARL by Novel Ketoamide Inhibitors Reveals Its Role in PINK1/Parkin-Dependent Mitophagy. J Med Chem. 2023 Jan 12;66(1):251-265. |