OSMI-3_product_DataBase

Bioactivity	OSMI-3 (Compound 2b) is a potent, long-lasting, and cell-permeable O-linked N-acetylglucosamine transferase (OGT) inhibitor. Cells contain a large nuclear pool of partially spliced OGT transcript, and OSMI-3 increases detained intron splicing in cells[1].
Target	O-linked N-acetylglucosamine transferase (OGT)
Invitro	OSMI-3 (Compound 2b; 20-50 μM; 4-24 hours; HCT116 cells) treatment significantly reduces O-GlcNAc levels, and has more sustained cellular effects than OSMI-2[1].A decrease in HCF-1 cleavage products and the appearance of uncleaved HCF-1 in cells treated with OSMI-3 (Compound 2b) is observed. Because OGT knockdown is known to decrease cell proliferation, the effects of OSMI-3 on cell growth in culture over 96h is also monitored. Although there is no evidence of apoptosis, the reduced growth of cells over time is observed, consistent with the knockdown results[1]. Western Blot Analysis[1] Cell Line:
Name	OSMI-3
CAS	2260791-13-5
Formula	C32H35N3O9S2
Molar Mass	669.77
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Martin SES, et al. Structure-Based Evolution of Low Nanomolar O-GlcNAc Transferase Inhibitors. J Am Chem Soc. 2018 Oct 24;140(42):13542-13545.

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