| Bioactivity | ODN 2336 is a A-Class CpG ODN (oligodeoxynucleotides), is a potent TLR9 agonist. ODN 2336 induces the production of IFN-α. ODN 2336 up-regulates the expression of IP-10 mRNA and IL-18 mRNA. ODN 2336 can be used as adjuvant of vaccines[1][2][3]. |
| Invitro | ODN 2336 (0.5 µM; 24, 48 h) up-regulates the expression of f CD69 and IFN-α[1].ODN 2336 (3 µg/ml; 4 h) stimulates strang transcription of IP-10 mRNA after 4 h in human PBMC[1].ODN 2336 (3 µg/ml; 2, 4, 16 h) up-regulates the expression of IL-18 mRNA at the 16 htime point[1].ODN 2336 (50 μg/mL; 20 h) with IL-3 (10 ng/mL) induces the production of IFN-α in the plasma of adult and cord blood[2].ODN 2336 (0.3125-5 µg/ml; 24 h) increases the production of FNA1 in a dose-dependent manner in human PBMCs[3]. |
| Name | ODN 2336 |
| CAS | 332956-64-6 |
| Sequence | DNA, d(G-sp-G-sp-G-G-A-C-G-A-C-G-T-C-G-T-G-G-sp-G-sp-G-sp-G-sp-G-sp-G) |
| Molar Mass | 6761.70 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| Reference | [1]. urk M, et al. C-Class CpG ODN: sequence requirements and characterization of immunostimulatory activities on mRNA level. Immunobiology. 2004;209(1-2):141-54. [2]. old MC, et al. Purified neonatal plasmacytoid dendritic cells overcome intrinsic maturation defect with TLR agonist stimulation. Pediatr Res. 2006 Jul;60(1):34-7. [3]. Hilbert T, et al. Beta2-adrenoceptor stimulation suppresses TLR9-dependent IFNA1 secretion in human peripheral blood mononuclear cells. PLoS One. 2013 May 28;8(5):e65024. |