| Bioactivity | NLRP3-IN-32 (compound 7a), a 3, 4-dihydronaphthalene-1(2H)-one derivative, is a potential NLRP3 inflammatory vesicles inhibitor. NLRP3-IN-32 can block the assembly and activation of NLRP3 inflammasome by down-regulating the expression of NLPR3 and apoptosis-associated speck-like protein containing a CARD (ASC), and inhibiting the production of reactive oxygen species (ROS) and other inflammatory mediators. NLRP3-IN-32 inhibits the phosphorylation of IκBα and NF-κB/p65 and the nuclear translocation of p65, thereby inhibiting NF-κB signaling[1]. |
| Invitro | NLRP3-IN-32 (compound 7a; 1.5, 3, 6 μM) 以剂量依赖性方式逆转 LPS (HY-D1056) 刺激 RAW246.7 巨噬细胞炎症因子 TNF-α、IL-6、IL-18 和 IL-1β 的释放[1]。NLRP3-IN-32 (1.5, 3, 6 μM) 以剂量依赖性清除细胞内 LPS 刺激的 ROS 和 NO 产生，抑制 NLRP3 炎症小泡的激活[1]。NLRP3-IN-32 (1.5, 3, 6 μM) 对 RAW246.7 细胞具有低毒性，不同浓度下凋亡率分别为 3.8%、5.6% 和 6.8%[1]。NLRP3-IN-32 (1.5, 3, 6 μM; 24 小时) 剂量依赖性抑制 LPS (1.0 μg/mL; 2 小时) 刺激的 IκBα 磷酸化和 p65 磷酸化，从而抑制 NF-κB 信号通路激活[1]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> NLRP3-IN-32 相关抗体: |
| Formula | C21H22BrN3O |
| Molar Mass | 412.32 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Wen-Xuan Li, et al. Discovery of anti-inflammatory agents from 3, 4-dihydronaphthalene-1(2H)-one derivatives by inhibiting NLRP3 inflammasome activation. Eur J Med Chem. 2024 Feb 29:268:116284. |