| Bioactivity | NAI-N3 is a RNA acylation reagent that enables RNA purification. NAI-N3 is a dual-function SHAPE (selective 2-hydroxyl acylation and profiling experiment) probe (RNA structure probe and enrichment)[1]. |
| Invitro | Living cells are treated with the icSHAPE (in vivo click selective 2-hydroxyl acylation and profiling experiment) chemical NAI-N3 followed by selective chemical enrichment of NAI-N3-modified RNA, which provides an improved signal-to-noise ratio compared with similar methods leveraging deep sequencing[1]. |
| Name | NAI-N3 |
| CAS | 1612756-29-2 |
| Formula | C10H8N6O |
| Molar Mass | 228.21 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| Reference | [1]. Flynn RA, et al. Transcriptome-wide interrogation of RNA secondary structure in living cells with icSHAPE. Nat Protoc. 2016 Feb;11(2):273-90. |