| Bioactivity | Mito-TRFS, the first off-on probe, is used to image the mitochondrial thioredoxin reductase (TrxR2) in live cells[1]. |
| Invitro | Mito-TRFS (10 μM) has the maximal absorption at ~375 nm in TE buffer (50 mM Tris-HCl and 1 mM EDTA, pH 7.4) at 37 °C[1]. Mito-TRFS itself has a strong emission at ~480 nm when excited at 375 nm in TE buffer (φ=0.34), but has weak emission when excited at 438 nm[1]. Immunofluorescence[1]. Cell Line: |
| Name | Mito-TRFS |
| CAS | 1859102-62-7 |
| Formula | C38H34IN2O5PS2 |
| Molar Mass | 820.70 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture) |
| Reference | [1]. Yaping Liu, et al. A small molecule probe reveals declined mitochondrial thioredoxin reductase activity in a Parkinson's disease model. Chem Commun (Camb). 2016 Feb 7;52(11):2296-9. |