| Bioactivity | Mag-Fura-2 tetrapotassium is a UV excitable rational fluorescent Mg2+/Ca2+ indicator (Ex=334-360 nm, Em=510 nm). Mag-Fura-2 tetrapotassium can be used for the determination of Mg2+ and Ca2+ concentrations[1][2]. |
| Invitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Monitoring of Ca2+ release[1]:1. Incubate dispersed parotid acinar cells with 8 µM Mag-fura-2 tetrapotassium for 30 min at 37℃. (acinar cells for example).2. Wash cells twice with fresh HBSS-H without BSA.3. Precoate cell adhesive Cell-Tak on sample chambers.4. Transfer the dye-loaded cells to the chambers and attach to the bottom.5. Mounte the sample chambers on the stage of an inverted microscope (equipped with a 40 × objective), wash with BSA-free HBSS-H and then with Mg2+/ATP-free ICM.6. Incubate acinar cells with Mg2+/ATP-free ICM containing 50 µg/mL saponin for 3-5 min at room temperature.7. Wash the cells with ICM containing Mg2+ and ATP, and incubate in the complete ICM for at least 5 min to allow complete filling of the intracellular Ca2+ stores.8. Alternately excite permeabilised cells, capture and digitise fluorescence emission at 510 nm by a digital imaging system (record the 344 nm/360 nm ratio every 20 s).Note: ICM (intracellular-like medium) containing 125 mM KCl, 19 mM NaCl, 10 mM HEPES (pH 7.3 with KOH), 3 mM ATP, 1.4 mM MgCl2, 0.33 mM CaCl2, and 1 mM EGTA. |
| Name | Mag-Fura-2 tetrapotassium |
| CAS | 132319-57-4 |
| Formula | C18H10K4N2O11 |
| Molar Mass | 586.67 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Tojyo Y, et al. Monitoring of Ca2+ release from intracellular stores in permeabilized rat parotid acinar cells using the fluorescent indicators Mag-fura-2 and calcium green C18. Biochem Biophys Res Commun. 1997 Nov 7;240(1):189-95. [2]. Dai LJ, et al. Intracellular Mg2+ and magnesium depletion in isolated renal thick ascending limb cells. J Clin Invest. 1991 Oct;88(4):1255-64. |
Mag-Fura-2 tetrapotassium
CAS: 132319-57-4 F: C18H10K4N2O11 W: 586.67
Mag-Fura-2 tetrapotassium is a UV excitable rational fluorescent Mg2+/Ca2+ indicator (Ex=334-360 nm, Em=510 nm). Mag-Fur
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This product is for research use only, not for human use. We do not sell to patients.