| Bioactivity | ML328 is a selective inhibitor of bacterial AddAB and RecBCD helicase-nucleases with IC50 values of 26 and 5.1 μM, respectively. ML328 is a gyrase inhibitor. ML328 strongly inhibits the growth of E. coli in the presence of phage. ML328 can be used for the research of bacterial infection[1][2]. |
| Invitro | ML328 (0.1-1000 μM) 具有 AddAB 和 RecBCD 核酸酶抑制活性，IC50 值分别为 26 和 5.1 μM [1]。ML328 对 RecBCD，RecF 和 RecE 通路特异性地抑制高频重组[1]。ML328 对 RecBCD 具有抑制作用，对 RecBCD 核酸酶、促进 Hfr 重组和噬菌体 λ 重组的 IC50 值分别为 3，0.3 和 5 μΜ[2]。ML328 抑制 E. coli RecBCD，E. coli AddAB，M. smeg AddAB 和 M. smeg RecBCD 的 IC50 值分别为 4.6，16，2.4 和 5.5 μM[2]。Ml328 (25 μΜ；2 h) 降低了 H2O2 诱导 E. coli 突变的频率[2]。Ml328 (25 μΜ；1 h) 降低 H2O2 诱导 E. coli 缬氨酸抗性 (valineR) 突变的频率[2]。ML328 (50 μΜ) 轻度抑制 E. coli 生长，但在噬菌体存在时显著抑制 E. coli 生长[2]。 |
| Name | ML328 |
| CAS | 634175-34-1 |
| Formula | C22H21F3N6O3S |
| Molar Mass | 506.50 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Amundsen SK, et al. Small-molecule inhibitors of bacterial AddAB and RecBCD helicase-nuclease DNA repair enzymes. ACS Chem Biol. 2012 May 18;7(5):879-91. [2]. SMITH GERALD R, et al. ANTIBIOTIC COMPOUNDS AND COMPOSITIONS, AND METHODS FOR IDENTIFICATION THEREOF. WO/2013/142628. 2014. |