| Bioactivity | MFI8 is a small molecule inhibitor of mitochondrial[1]. |
| Target | Mitofusin |
| Invitro | MFI8 (20 μM; 6 h) reduces significantly mitochondrial aspect ratio with an EC50 of 4.8 μM, and inhibits mitochondrial function via mitofusins and subsequently promoting mitochondrial fission[1].MFI8 possesses functional groups that meet the criteria of the pharmacophore model to interact with mitofusins and promotes mitochondrial fission[1].MFI8 binds to the HR2 domain of MFN2, modulates MFN conformation and complexes, and alters mitochondrial functionality[1].MFI8 (0-20 μM; 6 h) concentration-responsively increases caspase-3/7 activity, induces cytochrome c release and decreases membrane potential in a mitofusin-dependent manner. MFI8 also induces DNA damage and induces cell death in combination with BV6 SMAC (second mitochondria-derived activator of caspases) mimetic[1]. |
| Name | MFI8 |
| CAS | 694488-83-0 |
| Formula | C16H18ClNO |
| Molar Mass | 275.77 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
| Reference | [1]. Zacharioudakis E, et al. Modulating mitofusins to control mitochondrial function and signaling. Nat Commun. 2022 Jul 7;13(1):3775. |