| Bioactivity | Laccase-IN-3 (Compound 2b) is a laccase inhibitor (IC50 = 1.02= μM) with significant antifungal activity. Laccase-IN-3 shows superior inhibitory effect on Botryosphaeria dothidea (EC50 = 0.17 mg/L). Laccase-IN-3 effectively blocks the catalytic function of laccase by binding to its active center. Laccase-IN-3 also disrupts pathogen cell membrane integrity and increases ROS[1]. |
| Invitro | Laccase-IN-3 (25 mg/L) 对真菌活性的抑制率分别为 98.2% (B. dothidea), 92% (S. sclerotiorum), 93.9 % (F. graminearum), 53.1 % (B. cinerea), 59.9 % (P. capsici), 22.0 % (P. infestans), 81.5 % (P. nicotianae)[1]。Laccase-IN-3 对各种真菌的 ECsub>50 分别为 0.17 mg/L (B. dothidea), 2.55 mg/L (S. sclerotiorum), 2.03 mg/L (F. graminearum)[1]。Laccase-IN-3 (0.79-6.25 mg/L;) 通过破坏 B. dothidea 菌丝的形态来抑制真菌生长[1]。Laccase-IN-3 (10-25 mg/L; 120 min) 破坏了 B. dothidea 菌丝体细胞膜的完整性，并增加了细胞膜透性。降低了细胞外 pH 值,破坏细胞内环境的稳定性[1]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. 0 --> Laccase-IN-3 相关抗体: |
| In Vivo | Laccase-IN-3 (100,200 mg/L; 6 天) 展现出对感染 B. dothidea 的 apple 显著的保护活性（92.6% 和 86.8%）和治疗活性（85.0% 和 78.9%），均优于 Boscalid (HY-119976)[1]。 MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
| Formula | C14H15FN2O |
| Molar Mass | 246.28 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Jin DJ, et al. Design, synthesis, antifungal evaluation and mechanism study of novel norbornene derivatives as potential laccase inhibitors. Pest Manag Sci. 2024 Apr 16. |