Bioactivity | Gaussia luciferase (GLuc) is the smallest known naturally secreted luciferase enzyme (20 kD) in vivo. When conducting fluorescence reporter experiments with Gaussia luciferase, detection can be directly performed using cell culture supernatant. This luciferase enzyme catalyzes the oxidation of coelenterazine to emit light (480 nm) without requiring ATP. LZCap AG(3'Acm) GLuc mRNA is synthesized and purified in a one-step transcription process using T7 RNA polymerase and LZCap AG(3'Acm) cap analog from a linear template. This mRNA is suitable for experiments related to mRNA delivery, translation efficiency, and transfection efficiency. |
Transport | Room temperature in continental US; may vary elsewhere. |
Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |