| Bioactivity | Janelia Fluor® 635, SE (JF635, SE) is a red fluorogenic fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. JF635, SE can be used for live cell imaging[1]. Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. |
| Invitro | Maximum absorption wavelength (λabs)=635 nm, maximum emission wavelength (λem)=652 nm[1].JF635 can serve as a ligand for self-labeling tag (such as HaloTag and SNAP-tag). The SNAP-tag ligand of JF635 (29) effectively labels SNAP-tag fusions in cells and the JF635–HaloTag ligand (27) could be used in a two-color experiment with JF525-SNAP-tag ligand[1], |
| Name | Janelia Fluor® 635, SE |
| Formula | C33H29F2N3O6Si |
| Molar Mass | 629.68 |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Reference | [1]. Grimm JB, et al. A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat Methods. 2017;14(10):987-994. |
Janelia Fluor® 635, SE
CAS: F: C33H29F2N3O6Si W: 629.68
Janelia Fluor® 635, SE (JF635, SE) is a red fluorogenic fluorescent dye containing an NHS ester that can be conjugated
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