Janelia Fluor® 585, SE_product_DataBase

Bioactivity	Janelia Fluor? 585, SE (JF585, SE) is an orange fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. Janelia Fluor? 585, SE can be used immediately for structured illumination (SIM) and stimulated emission depletion (STED) imaging and could be converted to photoactivatable derivative for single-molecule localization microscopy (SMLM) experiments[1]. Janelia Fluor? products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. Storage: protect from light.
Invitro	Maximum absorption wavelength (λabs)=585 nm, maximum emission wavelength (λem)=609 nm[1].The JF585-SNAP-tag ligand (24) also functioned as a cellular label. The new HaloTag ligands derived from JF585 and JF635 show a high degree of chromogenicity and fluorogenicity, a critical parameter in advanced imaging experiments[1].
Name	Janelia Fluor® 585, SE
CAS	1811539-88-4
Formula	C34H27F4N3O6
Molar Mass	649.59
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Grimm JB, Muthusamy AK, Liang Y, et al. A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat Methods. 2017;14(10):987-994. doi:10.1038/nmeth.4403

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