| Bioactivity | Hexidium iodide, a fluorescent nucleic binding acid stain (excitation/emission ~ 518/600 nm), permeants to mammalian cells and selectively stains almost all gram-positive bacteria. Hexidium iodide can bind to the DNA of all bacteria after permeabilization by EDTA[1][2]. |
| Invitro | Hexidium iodide allows assessment of Gram status by differential absorption through bacterial cell walls, selectively staining gram-positive organisms in suspension[1]. |
| Name | Hexidium iodide |
| CAS | 211566-66-4 |
| Formula | C25H28IN3 |
| Molar Mass | 497.41 |
| Appearance | Solid |
| Transport | Room temperature in continental US; may vary elsewhere. |
| Storage | 4°C, sealed storage, away from moisture and light *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
| Reference | [1]. Shanmugam BK, et al. Biodegradation of tannery dye effluent using Fenton's reagent and bacterial consortium: A biocalorimetric investigation. J Environ Manage. 2019;242:106-113. [2]. Holm C, et al. A flow-cytometric gram-staining technique for milk-associated bacteria. Appl Environ Microbiol. 2003;69(5):2857-2863. |