| Bioactivity | HKGreen-4I is a highly sensitive green fluorescent probe for the specific detection of H2O2 in living cells with a maximum excitation wavelength and emission wavelength of 520 nm and 543 nm, respectively[2]. | ||||||||||||
| Invitro | 1. HKGreen-4I 工作液的配制1.1 制备储存液用 150 μL 无水 DMSO 稀释 1 mg HKGreen-4I 去配制 10 mM 的储备液。注：HKGreen-4I 储存液建议分装后于 -20℃ 或 -80℃ 避光保存。1.2 工作液的配制用预热好的无血清细胞培养基或 PBS 稀释储存液，配制成 1-10 μM 的 HKGreen-4I 工作液。注：请根据实际情况调整 HKGreen-4I 工作液浓度，且现用现配。2. 细胞染色 (悬浮细胞) 2.1 离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。细胞密度在 1×106/mL。2.2 加入 1 mL HKGreen-4I 工作液，室温孵育 5-30 分钟。2.3 400 g，离心 3-4 分钟，弃去上清。2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，使用荧光显微镜或流式细胞仪进行观察。3. 细胞染色 (贴壁细胞) 3.1 将贴壁细胞培养于无菌盖玻片上。3.2 从培养基中移出盖玻片，吸除多余培养基。3.3 加入 100 μL 染料工作液，轻轻晃动使其完全覆盖细胞，孵育 5-30 分钟。3.4 吸去染料工作液，用培养基洗 2-3 次，每次 5 分钟 ，使用荧光显微镜或流式细胞仪进行观察。 | ||||||||||||
| Name | HKGreen-4I | ||||||||||||
| CAS | 1448821-82-6 | ||||||||||||
| Formula | C36H30N2O11 | ||||||||||||
| Molar Mass | 666.63 | ||||||||||||
| Appearance | Solid | ||||||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | ||||||||||||
| Storage |
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| Reference | [1]. Trends Cell Biol. 2007 Sep;17(9):422-7. doi: 10.1016/j.tcb.2007.07.009. Epub 2007 Sep 4. [2]. Yang D, et, al. Diarylamine-based fluorogenic probes for detection of peroxynitrite. US9651528B2. [3]. Ye S, et, al. Tandem Payne/Dakin Reaction: A New Strategy for Hydrogen Peroxide Detection and Molecular Imaging. Angew Chem Int Ed Engl. 2018 Aug 6;57(32):10173-10177. |
HKGreen-4I
CAS: 1448821-82-6 F: C36H30N2O11 W: 666.63
HKGreen-4I is a highly sensitive green fluorescent probe for the specific detection of H2O2 in living cells with a maxim
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