Green DND-26_product_DataBase

Bioactivity

Green DND-26 is a green fluorescently labeled lysosomal probe with a maximum excitation/emission wavelength of 504/511 nm. The structure is composed of a fluorescein group and linked weak bases, which can freely cross the cell membrane and generally gather on spherical organelles. Green DND-26 is suitable for observing the internal biosynthesis and related pathogenesis of lysosomes[1].

Invitro

1. 工作液配置：1.1 将溶液恢复至温室温，瞬时离心使其集中至管底。1.2 利用培养基或合适的缓冲液 (如PBS) 将 1 mM 储存液稀释至工作浓度，推荐工作液浓度为 50-100 nM。注：请根据实际情况调整 Green DND-26 工作液浓度，且现用现配。2. 细胞染色2.1 悬浮细胞：离心收集细胞，加入 PBS 洗涤两次，每次 5 分钟。贴壁细胞：弃去培养基，加入胰蛋白酶消化细胞。离心弃去上清后，加入 PBS 洗涤两次，每次 5 分钟。2.2 加入 1 mL Green DND-26 工作液，室温孵育 30 分钟。2.3 400 g，4℃ 离心 3-4 分钟，弃去上清。2.4 加入 PBS 洗涤细胞两次，每次 5 分钟。2.5 用 1 mL 无血清培养基或 PBS 重悬细胞后，在荧光显微镜或流式细胞仪下检测。保存条件-20℃ 避光保存

Name

Green DND-26

CAS

220524-71-0

Formula

C18H25BF2N4O

Molar Mass

362.23

Appearance

Solid

Transport

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Reference

[1]. Sun B, et al. Acid-Activatable Transmorphic Peptide-Based Nanomaterials for Photodynamic Therapy. Angew Chem Int Ed Engl. 2020;59(46):20582-20588. [2]. Van der Velden JL, et al. LysoTracker is a marker of differentiated alveolar type II cells. Respir Res. 2013;14(1):123. Published 2013 Nov 11.

PeptideDB

Green DND-26

CAS: 220524-71-0 F: C18H25BF2N4O W: 362.23