| Bioactivity | Fluorescein tyramide is a green fluorescent reagent (λabs: 494 nm; λem: 517 nm). Fluorescein tyramide is widely used for tyramide signal amplification (TSA) with a low-abundance in IHC, ICC, in situ hybridization (FISH) and flow cytometry (FCM) applications[1][2]. | |||||||||
| Invitro | HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal. | |||||||||
| Name | Fluorescein tyramide | |||||||||
| CAS | 210236-90-1 | |||||||||
| Formula | C29H21NO7 | |||||||||
| Molar Mass | 495.49 | |||||||||
| Appearance | Solid | |||||||||
| Transport | Room temperature in continental US; may vary elsewhere. | |||||||||
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| Reference | [1]. A H Hopman, et al. Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification. J Histochem Cytochem. 1998 Jun;46(6):771-7. [2]. R P van Gijlswijk, et al. Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J Histochem Cytochem. 1997 Mar;45(3):375-82. |
Fluorescein tyramide
CAS: 210236-90-1 F: C29H21NO7 W: 495.49
Fluorescein tyramide is a green fluorescent reagent (λabs: 494 nm; λem: 517 nm).Fluorescein tyramide is widely used fo
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