Fluo-3FF AM

CAS: 348079-13-0 F: C50H46Cl2F2N2O23 W: 1151.80

Fluo-3FF AM is a low affinity (Kd = 42 μM) fluorescent Ca2+ sensitive indicator (Abs/Em = 462 nm/526 nm). Fluo-3FF AM i

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Bioactivity	Fluo-3FF AM is a low affinity (Kd = 42 μM) fluorescent Ca2+ sensitive indicator (Abs/Em = 462 nm/526 nm). Fluo-3FF AM is Mg2+ insensitive and relatively photostable. Fluo-3FF AM is an analog of Fluo-3FF AM. Fluo-3FF AM is essentially non-fluorescent, but exhibits a strong fluorescence enhancement upon entry into cells and binding to calcium.[1]
Invitro	1. Fluo-3FF AM 工作液的配制1.1 制备储存液用0.025% (w/v) 的 Pluronic F-127/二甲基亚砜溶液稀释 Fluo-3FF AM 去配制 1 mM 的储备液。注：Fluo-3FF AM 储存液建议分装后于 -20℃ 或 -80℃ 避光保存。1.2 工作液的配制用平衡缓冲溶液，配制成 5 μM 的 Fluo-3 AM 工作液。注：请根据实际情况调整 Fluo-3FF AM 工作液浓度，且现用现配。2.细胞染色2.1 将贴壁细胞培养于无菌盖玻片上。2.2 从培养基中移出盖玻片，吸除多余培养基。2.3 加入 100 μL 染料工作液，轻轻晃动使其完全覆盖细胞，孵育 60 分钟。2.4 吸去染料工作液，然后在 4◦C 的 PSS 中清洗 60 分钟。
Name	Fluo-3FF AM
CAS	348079-13-0
Formula	C50H46Cl2F2N2O23
Molar Mass	1151.80
Transport	Room temperature in continental US; may vary elsewhere.
Storage	Please store the product under the recommended conditions in the Certificate of Analysis.
Reference	[1]. Gordienko DV, et al. Regulation of muscarinic cationic current in myocytes from guinea-pig ileum by intracellular Ca2+ release: a central role of inositol 1,4,5-trisphosphate receptors. Cell Calcium. 2004 Nov;36(5):367-86. [2]. Young RC, et al. Focal sarcoplasmic reticulum calcium stores and diffuse inositol 1,4,5-trisphosphate and ryanodine receptors in human myometrium. Cell Calcium. 1999 Jul-Aug;26(1-2):69-75.